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Electrophoretic exclusion for the selective transport of small molecules
Author(s) -
Meighan Michelle M.,
Keebaugh Michael W.,
Quihuis Alicia M.,
Kenyon Stacy M.,
Hayes Mark A.
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900340
Subject(s) - electrophoresis , electric field , cationic polymerization , capillary action , chemistry , capillary electrophoresis , flow (mathematics) , voltage , chemical physics , size exclusion chromatography , molecule , analytical chemistry (journal) , chromatography , materials science , mechanics , physics , polymer chemistry , organic chemistry , quantum mechanics , composite material , enzyme
A novel method capable of differentiating and concentrating small molecules in bulk solution termed “electrophoretic exclusion” is described and experimentally investigated. In this technique, the hydrodynamic flow of the system is countered by the electrophoretic velocity to prevent a species from entering into the channel. The separation can be controlled by changing the flow rate or applied electric field in order to exclude certain species selectively while allowing others to pass through the capillary. Proof of principle studies employed a flow injection regime of the method and examined the exclusion of Methyl Violet dye in the presence of a neutral species. Methyl Violet was concentrated almost 40 times the background concentration in 30 s using 6 kV. Additionally, a threshold voltage necessary for exclusion was determined. The establishment of a threshold voltage enabled the differentiation of two similar cationic species: Methyl Green and Neutral Red.