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CE‐ESI‐TOF/MS for human growth hormone analysis
Author(s) -
Staub Aline,
Giraud Sylvain,
Saugy Martial,
Rudaz Serge,
Veuthey JeanLuc,
Schappler Julie
Publication year - 2010
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900315
Subject(s) - human growth hormone , recombinant dna , biomolecule , growth hormone , chemistry , chromatography , hormone , biochemistry , gene
CE is a powerful analytical tool used to separate intact biomolecules such as proteins. The coupling of CE with TOF/MS produces a very promising method that can be used to detect and identify proteins in different matrices. This paper describes an efficient, rapid, and simple CE‐ESI‐TOF/MS procedure for the analysis of endogenous human growth hormone and recombinant human growth hormone without sample preparation. Operational factors were optimized using an experimental design, and the method was successfully applied to distinguish human growth hormone and recombinant human growth hormone in unknown samples.