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Measurement of electrophoretic mobility of cardiomyocytes
Author(s) -
Zhou Ying,
Liu Jun,
Wang Jing,
Wang Yan,
Sun Pei
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900287
Subject(s) - electrophoresis , surface charge , biophysics , chemistry , sialic acid , ionic strength , transmembrane protein , neuraminidase , diffusion , adsorption , chromatography , analytical chemistry (journal) , biochemistry , biology , enzyme , organic chemistry , thermodynamics , physics , receptor , aqueous solution
Abstract The electrophoretic mobility (EPM) of rat cardiomyocytes with or without the treatment of neuraminidase was studied by cell electrophoresis. The EPM was found to change over a range from 0 to 8.67 μm s −1 /V cm −1 , depending on ionic strength, transmembrane potential, pH value, and/or surface charges. It is interesting that zero EPM was observed but reverse of the mobility was not. These results suggested that the negative charges carried on the cardiomyocyte surface might comprehensively consist of surface sialic acid, plasmalemma proteins, phospholipids, and transmembrane potential. The aberrant electrical double layer formed between the carried negative charges and adions had a big adsorption layer and a diffusion layer whose sizes changed circularly, making only negative charges be carried on the surface of living cardiomyocytes. The special structures on the surface of cardiomyocytes probably play a considerable role in the process of cardiac electrical activity.