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Determination of isocyanates by capillary electrophoresis with tris(2,2′‐bipyridine)ruthenium(II) electrochemiluminescence
Author(s) -
Li Haijuan,
Shi Lihong,
Liu Xiaoqing,
Niu Wenxin,
Xu Guobao
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900281
Subject(s) - isocyanate , derivatization , amine gas treating , electrochemiluminescence , chemistry , reagent , capillary electrophoresis , detection limit , ruthenium , analyte , derivative (finance) , tertiary amine , tris , chromatography , polymer chemistry , organic chemistry , mass spectrometry , polyurethane , catalysis , biochemistry , financial economics , economics
Abstract CE with tris(2,2′‐bipyridyl) ruthenium(II) (Ru(bpy) 3 2+ ) electrochemiluminescence (ECL) detection for the quantitative determination of isocyanates was first reported. Hexamethylene diisocyanate (HDI) and hexyl isocyanate (HI) were used as the model analytes. Commercially available N , N ‐diethyl‐ N ′‐methylethylenediamine was used as the derivatization reagent. It has both a secondary amine group and a tertiary amine group. The secondary amine group can quantitatively react with isocyanate group, and the tertiary amine group can react with Ru(bpy) 3 2+ to produce strong ECL signal for sensitive detection. The derivatization reaction was almost instantaneous and is much faster than other reported derivative reactions using other derivative reagents. The urea formed was stable. Linear calibration curve was obtained in the range from 0.01 to 10 μM for HDI, and 0.02 to 20 μM for hexyl isocyanate (HI). The detection limit is 0.01 μM for HDI and 0.02 μM for HI. The method is more sensitive than UV‐detection and electrochemical detection. For practical application, recovery higher than 90% for HDI and HI was obtained for foam sample.