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Chemoselective ligand patterning of electroactive surfaces using microfluidics
Author(s) -
Westcott Nathan P.,
Yousaf Muhammad N.
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900275
Subject(s) - microfluidics , hydroquinone , self assembled monolayer , monolayer , nanotechnology , ligand (biochemistry) , adhesion , biocompatible material , chemistry , materials science , biosensor , combinatorial chemistry , organic chemistry , receptor , biochemistry , biomedical engineering , medicine
To generate model substrates for cell adhesion, we have developed two different biocompatible strategies based on self‐assembled monolayers (SAMs) of alkanethiolates on gold terminated with latent ketones and aldehydes. Under spatial control, the hydroquinone and alcohol‐terminated SAMs can be oxidized to allow for oxyamine ligand patterning on the surface with microfluidic cassettes. These immobilization strategies were characterized by electrochemistry, fluorescence, and utilizing a cell adhesive peptide, cell patterns were generated.