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Study of metallothionein oxidation by using of chip CE
Author(s) -
Krizkova Sona,
Adam Vojtech,
Kizek Rene
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900226
Subject(s) - metallothionein , chip , chemistry , nanotechnology , biochemistry , materials science , computer science , gene , telecommunications
Metallothionein (MT) is a low‐molecular mass protein playing an essential role in homeostasis of heavy metals ions. We used a chip‐based CE for quantitative study of MT oxidation. After oxidation of MT by H 2 O 2 we observed marked decrease in peaks heights and shift of peaks positions to higher molecular mass, which corresponded with the time of the oxidation. Moreover, we observed that the proportion of high‐molecular forms of MT was markedly increased. The oxidative changes were successfully reversed by using of reducing agent prior to electrophoresis. Our method can reveal the stability MT aggregates, of which biological role still remains unclear.