Premium
Establishing the mitochondrial DNA D‐loop structure of Columba livia
Author(s) -
Tsai LiChin,
Lee James ChunI,
Liao ShuPing,
Weng LinHsiang,
Linacre Adrian,
Hsieh HsingMei
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900194
Subject(s) - d loop , mitochondrial dna , biology , indel , genetics , genotyping , loop (graph theory) , indel mutation , single nucleotide polymorphism , microbiology and biotechnology , genotype , gene , mathematics , combinatorics
The complete mitochondrial DNA D‐loop structure of pigeon ( Columba livia ) was established in this study. A strategy of amplifying three partial fragments of the D‐loop and then combing the three fragments to cover the full length of the D‐loop was adopted. Ten samples from pigeons were collected and were successfully amplified and sequenced. Repetitive sequences of a VNTR and an STR were both observed at the 3′‐end of D‐loop region. DNA sequence data revealed polymorphic sequences including indels, SNP, VNTR and STR within the D‐loop. The size of the D‐loop ranged from 1310 to 1327 bp from the initiation site of D‐loop to the site immediately upstream of the repeat sequences depending upon the number of insertions or deletions. Each sample could be distinguished based on four genotyping procedures; being indels, SNPs, VNTRs and STRs. The polymorphic nature of the D‐loop can be a valuable method for maternal identification and genetic linkage of pigeon in particular forensic science investigations.