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Study of acetylcholinesterase inhibitors using CE with contactless conductivity detection
Author(s) -
SchuchertShi Aiping,
Hauser Peter C.
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900184
Subject(s) - acetylthiocholine , paraoxon , derivatization , acetylcholinesterase , chemistry , conductivity , chromatography , enzyme , capillary electrophoresis , hydrolysis , capillary action , biochemistry , materials science , aché , high performance liquid chromatography , composite material
The hydrolysis of acetylcholine and acetylthiocholine as catalyzed by the enzyme acetylcholinesterase was monitored by CE with contactless conductivity detection by determining the acetate produced in the reaction. This approach eliminates the need for a color forming derivatization procedure. The effects of the three inhibitors galanthamine, hyperzine A and paraoxon on the enzyme kinetics could also be investigated by the new procedure and the IC 50 values were determined. The contactless conductivity detection was also found to be compatible with the electrophoretically mediated microanalyis approach, in which the enzymatic reaction is carried out directly inside the capillary prior to separation and quantification.