Premium
CE of dsDNA in low‐molecular‐weight polyethylene oxide solutions
Author(s) -
Pereira Fiona,
Hassard Stuart,
Hassard John,
deMello Andrew
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900144
Subject(s) - ethylene oxide , nucleic acid , cellulose , polymer , polyacrylamide , resolution (logic) , polyethylene oxide , materials science , coating , oxide , polyethylene , linear range , chromatography , chemistry , analytical chemistry (journal) , chemical engineering , polymer chemistry , detection limit , nanotechnology , organic chemistry , biochemistry , artificial intelligence , computer science , copolymer , engineering
To realise effective size separations of nucleic acid fragments using CE, gel‐based matrices are commonly employed. The separation of label‐free dsDNA ladders and plasmid fragments in an uncross‐linked semi‐dilute poly (ethylene) oxide solution using multi‐pixel UV detection at 254 nm is reported. Improvements in the sensitivity of UV detection of dsDNA using signal averaging over multiple pixels is demonstrated. Separations performed using a diode array detector also allow the progress of the separation to be monitored as a function of time. Several polymers were examined including methyl cellulose, linear polyacrylamide, hydroxy (propyl) methylcellulose and polyethylene oxide. Operations parameters investigated included UV transparency, self‐coating capacity and separation efficiency. The results show complete resolution of all fragments under a range of conditions, including short separation lengths.