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Photo‐polymerized lauryl methacrylate monolithic columns for CEC using lauroyl peroxide as initiator
Author(s) -
BernabéZafón Virginia,
BeneitoCambra Miriam,
SimóAlfonso Ernesto F.,
RamisRamos Guillermo,
HerreroMartínez José Manuel
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900038
Subject(s) - polymerization , alkylbenzenes , chemistry , monomer , peroxide , monolithic hplc column , van deemter equation , chromatography , monolith , methacrylate , benzoyl peroxide , organic peroxide , cationic polymerization , high performance liquid chromatography , polymer chemistry , copolymer , polymer , organic chemistry , catalysis
Lauryl methacrylate (LMA)‐ester based monolithic columns photo‐polymerized using lauroyl peroxide (LPO) as initiator were prepared, and their morphological and CEC properties were studied. The composition of the polymerization mixture ( i.e. ratios of monomers/porogenic solvents, 1,4‐butanediol/1‐propanol and LMA/crosslinker) was optimized. The morphological and chromatographic properties of LMA columns were evaluated by means of SEM pictures and van Deemter plots of PAHs, respectively. The polymerization mixture selected as optimal provided a fast separation of a mixture of PAHs with excellent efficiencies (minimum plate heights of 8.9–11.1 μm). Satisfactory column‐to‐column (RSD<4.5%) and batch‐to‐batch reproducibilities (RSD<6.3%) were achieved. The LMA columns photo‐polymerized with LPO were compared with those prepared with AIBN. Using PAHs, alkylbenzenes and basic compounds for testing, the columns obtained with LPO gave the best compromise between efficiency, resolution and analysis time.