Premium
Improved resolution in the acidic and basic region of 2‐DE of insect antennae proteins using hydroxyethyl disulfide
Author(s) -
Acín Patricia,
Rayó Josep,
Guerrero Angel,
Quero Carmen
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900031
Subject(s) - streaking , disulfide bond , buffer (optical fiber) , chemistry , reducing agent , resolution (logic) , chromatography , combinatorial chemistry , biochemistry , organic chemistry , biology , computer science , telecommunications , artificial intelligence , microbiology and biotechnology
Olfaction is essential for processing chemical signals in insects, but characterizing the proteins implicated in this process has proved challenging. We optimized 2‐DE gel resolution of insect proteins by using a buffer containing two reducing agents, DTT and hydroxyethyl disulfide. This buffer clearly improved resolution and decreased spot streaking and spot trains of 2‐DE in comparison to DTT alone. We described for the first time that the buffer with DTT and hydroxyethyl disulfide further to reducing streaking in the basic part of the gel eliminates false spots in the acidic gel regions that appeared when only DTT was used as reducing agent.