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Electrokinetic trapping using titania nanoporous membranes fabricated using sol–gel chemistry on microfluidic devices
Author(s) -
Hoeman Kurt W.,
Lange Jeffrey J.,
Roman Gregory T.,
Higgins Daniel A.,
Culbertson Christopher T.
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900027
Subject(s) - membrane , microfluidics , electrokinetic phenomena , analyte , nanoporous , materials science , vinyl alcohol , chemical engineering , nanotechnology , analytical chemistry (journal) , chromatography , chemistry , polymer , biochemistry , engineering , composite material
We have developed a new method for analyte preconcentration on a microfluidic device using a porous membrane fabricated via sol–gel chemistry. These porous membranes were fabricated within the channels of glass microfluidic devices exploiting laminar flow to bring an alcoholic sol–gel precursor (titanium isopropoxide in 2‐propanol) into contact with an alcohol–water solution at a channel cross intersection. These two streams reacted at the fluidic interface to form a porous titania membrane. The thickness of the membrane could be altered by changing the [H 2 O]. Analyte concentration was accomplished by applying a voltage across the titania membrane. The level of analyte enrichment was monitored, and enrichment factors of above 4000 in 400 s were obtained for 2,7‐dichlorofluorescein.