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Online purification and determination of β‐antagonists in blood sample based on heart‐cutting CE with electrochemical detection
Author(s) -
Zhang Zhaoxiang,
Zhang Mingzhe,
Zhang Shusheng
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900020
Subject(s) - chromatography , capillary electrophoresis , sample (material) , chemistry , capillary action , analytical chemistry (journal) , stacking , sample preparation , materials science , composite material , organic chemistry
A novel method to detect β‐antagonists in rat blood was developed by combining heart‐cutting 2‐D CE in a single capillary with on‐column electrochemical (EC) detection. The blood sample was purified in first dimension by non‐micellar CZE separation. Then only a desirable fraction of the first dimension separation was transferred into the second dimension of the capillary by pressure. Finally, the purified sample was separated by MEKC. On‐line dual concentration method, field amplified sample stacking and sweeping, was employed to accumulate the purified components that effectively counteracted the sample zone diffusion during the mobilization pressure step. A field decoupler joint and an EC detection cell were employed for on‐column EC detection. After optimizing the analytical conditions, the RSD of peak height, peak area and migration time were 2.1–4.3%, 1.5–3.9% and 0.7–1.8% ( n =10), respectively. This method has been successfully applied to determine β‐antagonists in postdosing rat blood sample. The pharmacokinetic study also demonstrated that the proposed heart‐cutting 2‐D CE with on‐column EC detection was convenient, sensitive and would become an attractively alternative method for on‐line sample cleanup and separation in complex samples.