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Carbon nanotube/poly(ethylene‐co‐vinyl acetate) composite electrode for capillary electrophoretic determination of esculin and esculetin in Cortex Fraxini
Author(s) -
Chen Zhi,
Zhang Luyan,
Chen Gang
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900019
Subject(s) - carbon nanotube , electrode , amperometry , capillary electrophoresis , materials science , composite number , ethylene vinyl acetate , capillary action , chemical engineering , microfluidics , vinyl acetate , ethylene , analytical chemistry (journal) , chromatography , electrochemistry , chemistry , nanotechnology , composite material , polymer , organic chemistry , catalysis , engineering , copolymer
In this report, a novel carbon nanotube/poly(ethylene‐co‐vinyl acetate) (CNT/EVA) composite electrode was developed for the amperometric detection in CE. The composite electrode was fabricated by packing a mixture of CNTs and melted EVA in a piece of fused‐silica capillary under heat. It was coupled with CE for the separation and detection of esculin and esculetin in Cortex Fraxini, a traditional Chinese medicine, to demonstrate its feasibility and performance. Esculin and esculetin have been well separated within 9 min in a 40 cm long capillary at a separation voltage of 12 kV using a 50 mM borate buffer (pH 9.2). The new CNT‐based CE detector offered significantly lower detection potentials, yielded enhanced S / N characteristics, and exhibited high resistance to surface fouling and enhanced stability. It showed long‐term stability and reproducibility with relative standard deviations of less than 5% for the peak current ( n =15) and should also find a wide range of applications in other microfluidic analysis systems.