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Determination of liposome–buffer distribution coefficients of charged drugs by capillary electrophoresis frontal analysis
Author(s) -
Franzen Ulrik,
Jorgensen Lene,
Larsen Claus,
Heegaard Niels H. H.,
Østergaard Jesper
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200900013
Subject(s) - liposome , chemistry , chromatography , capillary electrophoresis , aqueous solution , electrophoresis , analytical chemistry (journal) , organic chemistry , biochemistry
The potential of using CE frontal analysis (CE‐FA) to study the interactions between a range of charged low molecular weight drug substances and liposomes was evaluated. The liposomes used were net negatively charged and consisted of 2‐oleoyl‐1‐palmitoyl‐ sn ‐glycero‐3‐phosphocholine and 1,2‐dipalmitoyl‐ sn ‐glycero‐3‐phosphate monosodium salt in a ratio of 80/20 mol%. Apparent distribution coefficients ( D mem ), defined as the molar concentration of drug substance in the membrane phase divided by the molar concentration of drug substance in the aqueous phase, were successfully determined for six positively and eight negatively charged drug substances with log D mem ranging from 1.35 to 3.63. The extent of liposome–buffer distribution was found to be dependent on the drug concentration. The results obtained with the developed CE‐FA method were in good agreement with results obtained by equilibrium dialysis. Furthermore, the CE‐FA method was faster, less labor intensive and required smaller sample volumes (∼50 μL) compared with equilibrium dialysis. Thus, CE‐FA is an efficient and useful tool for the characterization of interactions between liposomes and low molecular weight drug substances.