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Analysis of melamine in milk powder by CZE using UV detection and hyphenation with ESI quadrupole/TOF MS detection
Author(s) -
Klampfl Christian W.,
Andersen Liisa,
Haunschmidt Manuela,
Himmelsbach Markus,
Buchberger Wolfgang
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800786
Subject(s) - melamine , chromatography , chemistry , detection limit , electrospray , dichloromethane , sample preparation , quadrupole , mass spectrometry , analytical chemistry (journal) , solvent , physics , organic chemistry , atomic physics
An easy to employ method for the determination of melamine in milk powder samples using CZE with either UV or MS detection is presented. The separation capabilities of CZE allowed keeping sample preparation rather simple. Before analysis, milk powder samples were dissolved in water, de‐fatted with dichloromethane and diluted with ACN to precipitate the protein, filtrated and injected without further pretreatment. The developed CZE method allowed the determination of melamine in milk powder down to a level of 0.50 mg/kg using UV detection and 0.25 mg/kg when ESI quadrupole/TOF MS was employed for detection.
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