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Separation of R‐form lipopolysaccharide and lipid A by CE–Fourier‐transform ion cyclotron resonance MS
Author(s) -
Hübner Göran,
Lindner Buko
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800754
Subject(s) - fourier transform ion cyclotron resonance , ion cyclotron resonance , fourier transform , cyclotron resonance , ion , nuclear magnetic resonance , selected ion monitoring , chemistry , mass spectrometry , fourier transform infrared spectroscopy , resonance (particle physics) , analytical chemistry (journal) , cyclotron , chromatography , physics , atomic physics , organic chemistry , optics , gas chromatography–mass spectrometry , quantum mechanics
Lipopolysaccharide (LPS) is the main component of the outer leaflet of the outer membrane of Gram‐negative bacteria. Native isolates of LPS comprise a high inherent heterogeneity. This paper presents the first application of CE to separate and online mass analyze the different constituents of underivatized R‐form LPS and lipid A with an FT ion cyclotron resonance mass spectrometer. A Beckman P/ACE MDQ and a home‐made CE instrument were coupled to the mass spectrometer using a sheath liquid interface. An optimized CE electrolyte based on water, isopropyl alcohol, triethylamine and acetic acid at pH 8.9 allowed the separation of molecular species even of complex mixtures to a large extent with observed detection limits of single constituents of the samples in the low femtomol range. Comparison of CE‐MS and MS measurements under equal conditions enabled the investigation of ion suppression effects in LPS samples.