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An N‐methylpolyvinylpyridinium cationic polymer for capillary coating in electrophoresis of proteins and peptides
Author(s) -
Sebastiano Roberto,
Mendieta Martha E.,
Contiello Nunzia,
Citterio Attilio,
Righetti Pier Giorgio
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800672
Subject(s) - cationic polymerization , coating , chemistry , capillary electrophoresis , chromatography , isoelectric point , polymer , electrophoresis , repeatability , derivative (finance) , isoelectric focusing , polymer chemistry , organic chemistry , financial economics , economics , enzyme
Abstract A novel cationic polymeric coating is here reported, consisting of a N‐methylpolyvinylpyridinium quaternary ion. This coating, combined with an isoelectric, aspartic acid as BGE, offers excellent separations of low‐ to high‐ M r proteins, of low‐ to high‐p I values, coupled to very high run‐to‐run repeatability. The importance of a hydrophilic coating is also illustrated, whereas the N‐methylpolyvinylpyridinium quaternary ion does not seem to adsorb even a large size protein as human albumin, separations are already distorted in the N‐ethyl derivative and totally ruined in the N‐octyl derivative, due to its high hydrophobicity.