Premium
Liquid‐phase‐based separation systems for depletion, prefractionation and enrichment of proteins in biological fluids for in‐depth proteomics analysis
Author(s) -
Jmeian Yazen,
El Rassi Ziad
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800639
Subject(s) - proteomics , chromatography , sample preparation , chemistry , abundance (ecology) , mass spectrometry , proteome , computational biology , biology , biochemistry , gene , fishery
This review article is concerned with (i) proteomic sample preparation including among other things the depletion of high‐abundance proteins and the concentration of low‐abundance proteins and (ii) their subsequent prefractionation for further analysis. Subjects (i) and (ii) are essentials for in‐depth proteomic analysis by multidimensional liquid‐phase separation techniques ( e.g . multidimensional electrophoresis and LC in various formats) followed by mass spectrometric identification and bioinformatic. More than 90 papers published in the period covering early 2000 to the present have been reviewed. It should be noted that this review article is by no means exhaustive, and the aim was to rather provide a concise description of the latest developments in the field.