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Filmy channel microchip with amperometric detection
Author(s) -
Wang Wei,
Fu Feng F u,
Xu Xueqin,
Lin JinMing,
Chen Guonan
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800638
Subject(s) - joule heating , materials science , reproducibility , sensitivity (control systems) , amperometry , detector , channel (broadcasting) , chemistry , chromatography , electrode , optics , composite material , electronic engineering , computer science , electrochemistry , computer network , physics , engineering
In this article, a new type of microchip with filmy channels and a sample‐injection fracture is introduced. Unlike commercial microchip, new microchip possessed filmy channel with width 2–3 mm. The effective cooling ability made filmy channel microchip restrain the generation of Joule heat even under electric field of 588 V/cm. Moreover, wider channel could be more easily modified to prevent the absorption of samples, load more samples and result in a higher sensitivity. Sample‐injection fracture was first applied to match the filmy channel in microchip. Equipped with an amperometric detector, the characteristics of the newly designed filmy channel microchip had been studied and the results showed that it had good reproducibility, higher sensitivity and excellent separation ability. The microchip was also applied to separate L ‐tryptophan's metabolites, namely 5‐hydroxy‐ L ‐tryptophan, 5‐hydroxytryptamine and 5‐hydroxy‐indole‐3‐acetic acid.