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Direct automatic determination of free and total anesthetic drugs in human plasma by use of a dual (microdialysis–microextraction by packed sorbent) sample treatment coupled at‐line to NACE–MS
Author(s) -
MoralesCid Gabriel,
Cárdenas Soledad,
Simonet Bartolomé M.,
Valcárcel Miguel
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800493
Subject(s) - microdialysis , chromatography , sorbent , chemistry , anesthetic , solid phase microextraction , medicine , anesthesia , gas chromatography–mass spectrometry , mass spectrometry , biochemistry , extracellular , organic chemistry , adsorption
This paper reports for the first time the use of microextraction by packed sorbent in combination with CE. The combined system was used to determine anesthetic drugs in human plasma. A microdialysis fiber was coupled on‐line to the microextraction unit in order to distinguish between free and total concentrations of drugs. The system was automated by connecting the microextraction unit to a syringe pump and interfacing it to a computer. The ensuing method allows the determination of 10 μg/L concentrations of free drugs and 1 μg/L concentrations of total drugs from only 200 μL of sample with an RSD of less than 9%.