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Development of an in‐capillary derivatization method by CE for the determination of chiral amino acids in dietary supplements and wines
Author(s) -
MartínezGirón Ana Belén,
GarcíaRuiz Carmen,
Crego Antonio L.,
Marina Maria Luisa
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800481
Subject(s) - derivatization , chemistry , chromatography , capillary electrophoresis , amino acid , capillary action , mass spectrometry , biochemistry , materials science , composite material
A fast in‐capillary derivatization method by CE with 6‐aminoquinolyl‐ N ‐hydroxysuccinimidyl carbamate was developed for the first time for the determination of amino acid enantiomers (arginine, lysine, and ornithine) in dietary supplements and wines. Because of the initial current problems due to the formation of precipitates into the capillary during the derivatization reaction, a washing step with an organic solvent as DMSO between injections was necessary. Different approaches were also investigated to enhance the sensitivity of detection. A derivatization procedure, where plugs of ACN, derivatizing agent (10 mM 6‐aminoquinolyl‐ N ‐hydroxysuccinimidyl carbamate), and sample in borate (1:1 v/v) were injected in tandem (2, 3, and 6 s, respectively, at 50 mbar), was selected because it enabled to obtain the most sensitive and reproducible results. Appropriate analytical characteristics (linearity, LOD and LOQ, precision, absence of matrix interferences, and accuracy) were obtained for this method. Finally, the optimized method was successfully applied to the determination of the enantiomers of arginine, lysine, and ornithine in food samples of different complexities (dietary supplements and wines).