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A novel, environmentally friendly sodium lauryl ether sulfate‐, cocamidopropyl betaine‐, cocamide monoethanolamine‐containing buffer for MEKC on microfluidic devices
Author(s) -
Hoeman Kurt W.,
Culbertson Christopher T.
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800463
Subject(s) - chemistry , pulmonary surfactant , sodium dodecyl sulfate , micelle , buffer (optical fiber) , chromatography , betaine , ether , environmentally friendly , sodium , buffer solution , organic chemistry , aqueous solution , telecommunications , ecology , biochemistry , computer science , biology
A new buffer has been developed for fast, high‐efficiency separations of amino acids by MEKC. This buffer was more environmentally friendly than the most commonly used surfactant‐containing buffers for MEKC separations. It used a commercially available dishwashing soap by Seventh Generation™ (Burlington, VT, USA), which contained three micelle‐forming agents. The mixed micelles were composed of sodium lauryl ether sulfate (anionic), cocamidopropyl betaine (zwitterionic), and cocamide monoethanolamine (non‐ionic). The optimized buffer contained 5.0% w/w Seventh Generation™ Free & Clear™ dishwashing soap, 10 mM sodium borate, and was completely void of organics. The lack of organics and the biodegradability of the surfactant molecules made this buffer more environmentally friendly than typical SDS‐containing buffers. This new buffer also had a different selectivity and provided faster separations with higher separation efficiencies than SDS‐based buffers. Fast separations of BODIPY FL labeled amino acids yielded peaks with separation efficiencies greater than 100 000 in less than 20 s.