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On‐chip tryptic digest with direct coupling to ESI‐MS using magnetic particles
Author(s) -
Le Nel Anne,
Krenkova Jana,
Kleparnik Karel,
Smadja Claire,
Taverna Myriam,
Viovy JeanLouis,
Foret Frantisek
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800431
Subject(s) - microfluidic chip , chip , coupling (piping) , microfluidics , chemistry , chromatography , inductive coupling , mass spectrometry , materials science , analytical chemistry (journal) , nanotechnology , computer science , physics , telecommunications , quantum mechanics , metallurgy
As a step toward a fully automated front‐end microfluidic chip for MS proteomics, we propose a system capable of performing online tryptic digest and ESI‐MS, using a replaceable on‐chip digestion microcolumn based on self‐assembled magnetic particles.