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Application of Click‐chemistry‐based perphenylcarbamated β‐CD chiral stationary phase in CEC
Author(s) -
Wang Yong,
Xiao Yin,
Tan Timothy Thatt Yang,
Ng SiuChoon
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800424
Subject(s) - click chemistry , chemistry , analyte , aqueous solution , resolution (logic) , chiral stationary phase , phase (matter) , analytical chemistry (journal) , combinatorial chemistry , chromatography , organic chemistry , artificial intelligence , computer science
A novel chiral stationary phase (CSP) was prepared by anchoring mono‐6‐azido‐6‐deoxyperphenylcarbamated β‐CD onto ω‐alkynyl functionalized silica (5 μm) via organic soluble Cu(I)‐catalyzed Click chemistry . The obtained CSP was thereafter packed into fused‐silica capillary (100 μm id) with an effective length of 9 cm and tested in aqueous CEC to separate a series of racemic aryl alcohols. High separation factors with good resolution were achieved using the current novel CSP. Some pharmaceutical compounds could also be well resolved on this newly derived CSP. The analytical results demonstrate that Click ‐ chemistry ‐based perphenylcarbamated CSP affords high stability in high electric field and depicts excellent enantioseparation in CEC. The effects of pH value, buffer concentration, applied voltage, concentration of organic modifier and analyte structure on the enantioseparation are also discussed.