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Principles for different modes of multiple‐injection CZE
Author(s) -
Amini Ahmad,
Lodén Henrik,
Pettersson Curt,
Arvidsson Torbjörn
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800398
Subject(s) - spark plug , analyte , capillary electrophoresis , chromatography , analytical chemistry (journal) , capillary action , electrophoresis , chemistry , sample (material) , materials science , physics , thermodynamics , composite material
This paper introduces four different modes of multiple‐injection CZE (MICZE). The validity of these MICZE models was evaluated by the experimental data. Prior to the application of MICZE, the electrophoretic conditions are developed in the single‐injection mode by adjusting different experimental parameters such as pH, type and concentration of buffer additives and temperature. Based on the migration time difference (Δ t mig ) between the analyte and the internal standard or injection marker, one or more MICZE modes can be employed. The injection marker is added to the sample to compensate for injection‐volume fluctuations. The inter‐plug distance is regulated by applying an electrical field over the capillary for a short period of time between each injection. After the final injection, the separation is completed by electrophoresis for a time period corresponding to that in the single‐injection mode.