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Large volume stacking using an EOF pump in NACE‐MS
Author(s) -
Kim Jihye,
Chun ManSeog,
Choi Kihwan,
Chung Doo Soo
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800396
Subject(s) - stacking , spark plug , capillary action , analyte , chromatography , analytical chemistry (journal) , backup , buffer (optical fiber) , volume (thermodynamics) , chemistry , matrix (chemical analysis) , solvent , materials science , computer science , composite material , telecommunications , physics , organic chemistry , quantum mechanics , engineering , aerospace engineering , database
Large volume stacking using an EOF pump (LVSEP) is realized for CE with ESI‐MS. In LVSEP of anions, the adverse effects of a long sample matrix plug were overcome by pumping the plug out of the separation capillary to the inlet vial using the EOF under a potential of reverse polarity. Therefore, it was necessary to supply a backup run buffer from the outlet end of the capillary, which was not trivial in most ESI‐MS configurations. We solved this difficulty simply by placing a vial supplying a backup run buffer during the matrix removal and sample stacking process. In addition, methanol was used as the run buffer solvent enabling a bare fused‐silica capillary to meet the requirement of suppressed EOF. This LVSEP CE/ESI‐MS has been successfully applied to six anionic analytes, achieving 400‐fold enrichment in the detection sensitivity by using a conventional sheath liquid CE/ESI‐MS interface without any physical modification.