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Development of a non‐aqueous electrophoresis method for the simultaneous determination of tricyclic antidepressants in human serum
Author(s) -
AcedoValenzuela MaríaIsabel,
GaleanoDíaz Teresa,
Rodríguez Antonio Silva,
Martínez Rita Carabias,
Álvarez Javier Domínguez
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800383
Subject(s) - chromatography , acetic acid , ammonium acetate , chemistry , electrokinetic phenomena , aqueous solution , analyte , tricyclic , resolution (logic) , ethyl acetate , detection limit , electrophoresis , calibration curve , matrix (chemical analysis) , standard addition , high performance liquid chromatography , organic chemistry , artificial intelligence , computer science
In the present work we report a non‐aqueous electrophoresis method (NACE) for the separation and determination of eight tricyclic antidepressants (TCAs) in human serum. Separation is carried out in 1 M acetic acid, 50 mM ammonium acetate and 10 mM SDS in ACN. Standards and samples were prepared in 0.8 M acetic acid in ACN and introduced by electrokinetic injection. The interactions between TCAs and acetate ions were studied and deduced from comparison of both the actual experimental and theoretical mobility. The establishment of calibration curves in the presence or absence of serum matrix, between 20 and 200 ng/mL, together with the determination of most important analytical parameters and a study of robustness were performed. Validation of the CE method was carried out with serum samples spiked with the analytes. The NACE determination method offers better resolution and higher sensitivity than the determination of TCAs in aqueous media.