Premium
Adapted liquid crystal display backlighting unit for densitometry of stained polyacrylamide electrophoresis gels
Author(s) -
Tan Han Yen,
Ng Tuck Wah,
Liew Oi Wah
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800363
Subject(s) - densitometry , monochromatic color , polyacrylamide , backlight , electrophoresis , repeatability , chromatography , materials science , polyacrylamide gel electrophoresis , liquid crystal display , optics , chemistry , optoelectronics , biochemistry , physics , polymer chemistry , enzyme
Appropriate monochromatic illumination that is spatially uniform will ensure densitometry of stained polyacrylamide electrophoresis gels with high sensitivity and repeatability. The approach of using an array of LEDs as an illuminator has practical limitations. Here we describe an alternative method based on adapted liquid crystal display backlighting. By changing the fluorescent light source and using a region where light intensity is uniform, we demonstrate the ability to achieve highly sensitive and repeatable densitometry measurements of polyacrylamide electrophoresis gels with Coomassie‐stained proteins.