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Modified cyclodextrins for fast and sensitive chiral‐capillary electrophoresis‐mass spectrometry
Author(s) -
Giuffrida Alessandro,
León Carlos,
GarcíaCañas Virginia,
Cucinotta Vincenzo,
Cifuentes Alejandro
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800333
Subject(s) - capillary electrophoresis , mass spectrometry , chromatography , capillary electrophoresis–mass spectrometry , chemistry , capillary action , analytical chemistry (journal) , materials science , electrospray ionization , composite material
Abstract In this work, three modified CDs (mCDs), namely 6‐deoxy‐6‐[1‐(2‐amino)ethylamino]‐β‐CD, 6‐deoxy‐6‐[ N ‐(2‐methylamino)pyridine)]‐β‐CD and 3‐monodeoxy‐3‐monoamino‐β‐CD, are investigated as chiral selectors for CE with LIF (CE‐LIF) and CE‐TOF‐MS. The potential of these three mCDs as chiral selectors in CE was also compared with the unmodified β‐CD and γ‐CD. A group of ten different D ‐ and L ‐amino acids derivatized with FITC was selected as case‐study for CE‐LIF and CE‐MS comparison. The analyte group includes negatively ( D / L ‐Glu and D / L ‐Asp), neutral ( D / L ‐Ala and D / L ‐Asn) and positively ( D / L ‐Arg) charged amino acids. Chiral separation conditions compatible with CE‐TOF‐MS could be achieved based on the figures of merit obtained by CE‐LIF using the different CDs. Interestingly, LOD values obtained by chiral CE‐TOF‐MS were in the nM range comparable or only slightly worse to those obtained by CE‐LIF. Moreover, the time of analysis using mCDs was reduced more than 30% compared with the non‐chiral conditions (19.2 versus 28.4 min, respectively), with resolution and efficiency values as high as 5.15 and 843 000 plates/m, respectively. The usefulness of this chiral CE‐TOF‐MS method was corroborated through the detection of the main D ‐ and L ‐amino acids found in different real samples including transgenic versus wild soy and vinegar.