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Screening and confirmatory methods for the analysis of macrocyclic lactone mycotoxins by CE with amperometric detection
Author(s) -
Arribas Alberto Sánchez,
Bermejo Esperanza,
Zapardiel Antonio,
Téllez Helena,
RodríguezFlores Juana,
Zougagh Mohammed,
Ríos Ángel,
Chicharro Manuel
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800305
Subject(s) - chromatography , analyte , detection limit , amperometry , zearalenone , chemistry , mycotoxin , extraction (chemistry) , electrode , food science , electrochemistry
A simple analytical scheme for the screening and quantification of zearalenone and its metabolites, α‐zearalenol and β‐zearalenol, is reported. Extracts from maize flour samples were collected by supercritical fluid extraction and afterwards, they were analyzed by CE with amperometric detection. This scheme allowed a rapid and reliable identification of contaminated flour samples according to the reference value established for zearalenone by directive 2005/38/EC (200 μg/kg). The sample screening method was carried out by CZE using 25 mM borate separation buffer at pH 9.2 and 25.0 kV as separation voltage, monitoring the amperometric signal at +700 mV with a carbon paste electrode. In this way, total amount of mycotoxins was determined and samples were processed in 4 min with a detection limit of 12 μg/L, enough to discriminate between positive (more than 200 μg/L total mycotoxins) and negative samples (less than 200 μg/L total mycotoxins). Positive samples were then subjected to CZE separation and quantification of each analyte was done with 50 mM borate running buffer modified with 30% methanol at pH 9.7 and 17.5 kV as separation voltage. Under these conditions, separation was achieved in 15 min with detection limits from 20 to 35 μg/L for each analyte.