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Internally calibrated quantification of protein analytes in human serum by fluorescence immunoassays in disposable elastomeric microfluidic devices
Author(s) -
Kartalov Emil P.,
Lin David H.,
Lee David T.,
Anderson William F.,
Taylor Clive R.,
Scherer Axel
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800297
Subject(s) - analyte , microfluidics , chromatography , immunoassay , calibration , point of care testing , fluorescence , materials science , nanotechnology , chemistry , biomedical engineering , biology , engineering , immunology , statistics , mathematics , physics , antibody , quantum mechanics
Herein we report on reliable reproducible quantification of protein analytes in human serum by fluorescence sandwich immunoassays in disposable PDMS microfluidic chips. The system requires 1000 times less sample than typical clinical blood tests and is specifically shown to measure ferritin down to 250 pM in human serum. The in‐built calibration method of spiking the serum with known concentrations of commercially available antigen avoids common sources of error and improves the reliability of the test results. The reported microfluidic system is an important new tool for fundamental scientific research, offering sensitive immunoassay measurements in small but complex biosamples. The system is also a further step towards comprehensive affordable “point‐of‐care” biomedical diagnostics.