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Chip‐CE/MS using a flat low‐sheath‐flow interface
Author(s) -
Li FuAn,
Huang JuLi,
Her GuorRong
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800271
Subject(s) - capillary action , common emitter , body orifice , materials science , chip , sprayer , epoxy , flow (mathematics) , analytical chemistry (journal) , optoelectronics , chemistry , composite material , chromatography , mechanical engineering , electrical engineering , mechanics , physics , engineering
A chip‐CE/ESI/MS interface based on a low‐sheath‐flow design has been developed. A flat low‐sheath‐flow interface was fabricated to facilitate the coupling with a CE microchip. The interface consists of a PMMA reservoir block, a PMMA platform and a replaceable ESI sprayer. A CE interface was constructed by using a wire‐assisted epoxy‐fixing method to connect a 1.5 cm connecting capillary to the end of chip‐CE channel. The opposite end of the connecting capillary was tapered to approximately 40 μm od to fit tightly inside the back end of a removable fused‐silica capillary ESI sprayer, which was also tapered to give a 10 μm orifice. With this 1.5 cm connecting capillary, the sheath liquid flowed coaxially around the connecting capillary to create a low dead volume liquid junction at the interface between the connecting capillary and the ESI emitter. An advantage of the current design over existing chip‐based CE/MS interfaces is that ESI emitter can easily be replaced. The analytical utility of this microdevice was demonstrated by the analysis of two synthetic mixtures: a series histamine antagonists and a mixture of synthetic peptides.