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Evaluation of gel electrophoresis conditions for the separation of metal‐tagged proteins with subsequent laser ablation ICP‐MS detection
Author(s) -
Raab Andrea,
Pioselli Barbara,
Munro Caroline,
ThomasOates Jane,
Feldmann Jörg
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800264
Subject(s) - myoglobin , chemistry , chromatography , metal , gel electrophoresis , electrophoresis , transferrin , metalloprotein , biochemistry , organic chemistry
Although laser ablation (LA)‐ICP‐MS has been reported for the determination of metalloproteins separated by gel electrophoretic techniques (GE), systematic studies that define the conditions essential for successful measurements are still scarce. In this paper we present the results of our studies of basic conditions for the effective application of GE‐LA‐ICP‐MS for the separation of metal‐binding proteins, focusing on their stability during GE and post‐separation gel treatment. The stability of metal–protein complexes (haemoglobin, myoglobin, superoxide dismutase, carbonic anhydrase, transferrin, albumin, cytochrome c ) during GE is dependent on the nature of the metal–protein interaction and the principle of separation. We have observed that non‐denaturing GE is a suitable separation technique for most metal–protein complexes ( e.g . Zn in carbonic anhydrase and Fe in Tf and myoglobin were quantitatively recovered in a spiked liver cytosol), whereas separation by denaturing GE strongly impaired the stability of the complexes. Equally important is the post‐separation treatment of the gel to enable successful detection of the metal. LA‐ICP‐MS requires drying of the gel without loss of protein‐bound metal or cracking of the gel. This was successfully achieved using glycerol followed by heating. We demonstrate that staining of the gel prior to LA‐ICP‐MS using silver or Coomassie blue is not recommended, since most protein‐bound metal is lost during the staining procedure. Furthermore it has been shown that only line scanning with a speed of less than 30 μm/s can reliably distinguish between lines 1 mm apart, while raster spot analysis carries the risk of misinterpretation due to contamination in/on inhomogeneous gels.