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Development of an acrylate monolith in a cyclo‐olefin copolymer microfluidic device for chip electrochromatography separation
Author(s) -
Faure Karine,
Albert Monique,
Dugas Vincent,
Crétier Gérard,
Ferrigno Rosaria,
Morin Pierre,
Rocca JeanLouis
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800235
Subject(s) - monolith , capillary electrochromatography , copolymer , acrylate , materials science , microfluidics , electrochromatography , methacrylate , chemical engineering , butyl acrylate , olefin fiber , polymer , capillary action , chromatography , chemistry , nanotechnology , composite material , organic chemistry , catalysis , engineering
An acrylate monolith has been synthesized into a cyclic olefin copolymer microdevice for reversed‐phase electrochromatography purposes. Microchannels, designed by hot embossing, were filled up with an acrylate monolith to serve as a hydrophobic stationary phase. A lauryl acrylate monolith was formulated to suit the hydrophobic material, by implementing 100% organic porogenic solvent. This new composition was tested in capillary prior to its transfer into the microfluidic device. Surface functionalization of the cyclic olefin copolymer surface was applied using UV‐grafting technique to improve the covalent attachment of this monolith to the plastic walls of the microfluidic chip. The on‐chip performances of this monolith were evaluated in detail for the reversed‐phase electrochromatographic separation of polycyclic aromatic hydrocarbons, with plate heights reaching down to 10 μm when working at optimal velocity.