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Assessment of CE for the identification of microorganisms
Author(s) -
Petr Jan,
Ryparová Olga,
Ranc Václav,
Hinnerová Petra,
Znaleziona Joanna,
Kowalska Marta,
Knob Radim,
Maier Vítězslav,
Frébort Ivo,
Lemr Karel,
Ševčík Juraj
Publication year - 2009
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800232
Subject(s) - microorganism , identification (biology) , computational biology , biochemical engineering , chemistry , environmental chemistry , biology , microbiology and biotechnology , bacteria , engineering , genetics , botany
The assessment of capillary electrophoresis for separation and identification of microorganisms is described in this article. The work brings a comparison of the application of uncoated capillaries modified with poly(ethylene oxide) and coated capillaries for the separation of model microorganisms Saccharomyces cerevisiae and Escherichia coli with Tris–borate–EDTA and Tris–citrate–fructose as electrolytes. The best separation was achieved in the coated capillary using 1 mM Tris–citrate–fructose buffer, pH 6.9. A simple identification based on the migration time and UV spectrum was found unsatisfactory and thus a method based on a post‐separation cultivation and sequential analysis was developed. Off‐line combination with mass spectrometric analysis with the use of desorption electrospray was shown to be an interesting alternative in a study of microorganisms. Mass spectra allowed distinguishing among analyzed cells.