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Dielectrophoretic characterization of erythrocytes: Positive ABO blood types
Author(s) -
Srivastava Soumya K.,
Daggolu Prashant R.,
Burgess Shane C.,
Minerick Adrienne R.
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800166
Subject(s) - abo blood group system , dielectrophoresis , blood typing , red blood cell , blood type (non human) , electrophoresis , blood cell , typing , whole blood , electrode , rh blood group system , chemistry , biomedical engineering , materials science , chromatography , biology , biochemistry , immunology , medicine , genetics , antibody
Dielectrophoretic manipulation of erythrocytes/red blood cells is investigated as a tool to identify blood type for medical diagnostic applications. Positive blood types of the ABO typing system (A+, B+, AB+ and O+) were tested and cell responses quantified. The dielectrophoretic response of each blood type was observed in a platinum electrode microdevice, delivering a field of 0.025 V pp /μm at 1 MHz. Responses were recorded via video microscopy for 120 s and erythrocyte positions were tabulated at 20–30 s intervals. Both vertical and horizontal motions of erythrocytes were quantified via image object recognition, object tracking in MATLAB, binning into appropriate electric field contoured regions (wedges) and statistical analysis. Cells of O+ type showed relatively attenuated response to the dielectrophoretic field and were distinguished with greater than 95% confidence from all the other three blood types. AB+ cell responses differed from A+ and B+ blood types likely because AB+ erythrocytes express both the A and B glycoforms on their membrane. This research suggests that dielectrophoresis of untreated erythrocytes beyond simple dilution depends on blood type and could be used in portable blood typing devices.