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Preparation and characterization of hexyl methacrylate monolithic columns for CEC
Author(s) -
CantóMirapeix Amparo,
HerreroMartínez José M.,
MongayFernández Carlos,
SimóAlfonso Ernesto F.
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800154
Subject(s) - aqueous solution , methacrylate , solvent , polymerization , chemistry , repeatability , chromatography , ammonium , polymer , organic chemistry
The preparation of hexyl methacrylate (HMA) monolithic columns for CEC separations has been investigated with two initiation systems: (i) ammonium peroxodisulphate and TEMED to activate the polymerization reaction, and (ii) by thermal initiation with AIBN. For both initiators, the influence of composition of porogenic solvent on morphological and chromatographic properties of monoliths was investigated. Two porogenic solvent systems, aqueous and non‐aqueous media, were also studied for monolithic beds polymerized with AIBN. Under optimal conditions, low minimum plate heights (9.6 μm for peroxodisulphate, 8.4 and 10.0 μm for AIBN in aqueous and non‐aqueous porogenic solvents, respectively) were obtained. A comparison in terms of chromatographic performance of HMA monoliths with butyl methacrylate columns polymerized with both initiators was also performed. The resulting HMA‐based stationary phases also exhibited a good repeatability and column‐to‐column reproducibility, with RSD values below 5.6% in the studied electrochromatographic parameters. The potential of HMA‐based columns was demonstrated by the analysis of complex mixtures of polyaromatic hydrocarbons and anabolic steroids.