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Retention of proteins and metalloproteins in open tubular capillary electrochromatography with etched chemically modified columns
Author(s) -
Pesek Joseph J.,
Matyska Maria T.,
Salgotra Vasudha
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800142
Subject(s) - capillary electrochromatography , metalloprotein , chemistry , electrochromatography , chromatography , capillary action , capillary electrophoresis , materials science , biochemistry , enzyme , composite material
Etched chemically modified capillaries with two different bonded groups (pentyl and octadecyl) are compared for their migration behavior of several common proteins and metalloproteins as well as metalloproteinases. Migration times, efficiency and peak shape are evaluated over the pH range of 2.1–8.1 to determine any effects of the bonded group on the electrochromatographic behavior of these compounds. One goal was to determine if the relative hydrophobicity of the stationary phase has a significant effect on proteins in the open tubular format of capillary electrochromatography as it does in HPLC. Reproducibility of the migration times is also investigated.