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New applications and developments in the use of multiplex ligation‐dependent probe amplification
Author(s) -
Kozlowski Piotr,
Jasinska Anna J.,
Kwiatkowski David J.
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800126
Subject(s) - multiplex ligation dependent probe amplification , multiplex , copy number variation , genotyping , computational biology , gene dosage , copy number analysis , biology , genetics , genome , gene , genotype , gene expression , exon
Multiplex ligation‐dependent probe amplification (MLPA) is a commonly used technique for determining relative DNA sequence dosage (or copy number) in a complex DNA sample. Originally MLPA was designed as a copy number analysis tool for detecting disease‐causing genomic mutations and has been successfully applied in the testing and identification of hundreds of genomic mutations in numerous genes including DMD , BRCA1 , NF1 , and TSC2 . More recently, several modifications of the original technique have been implemented. Arguably the most important enhancement of MLPA has been probe generation by chemical synthesis, enabling the facile creation of novel probe sets for any desired application. Other newer applications of MLPA include methylation status determination, copy number analysis in segmentally duplicated regions, expression profiling, and transgene genotyping. MLPA has a potential major role in the analysis of common copy number variation in genome‐wide association analyses, which may be enhanced by future improvements to increase throughput and lower costs, such as array‐MLPA.