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Glycosylation analysis of glycoproteins and proteoglycans using capillary electrophoresis‐mass spectrometry strategies
Author(s) -
Amon Sabine,
Zamfir Alina D.,
Rizzi Andreas
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800105
Subject(s) - glycoproteomics , glycan , context (archaeology) , glycosylation , chemistry , glycoprotein , mass spectrometry , capillary electrophoresis , proteomics , chromatography , computational biology , biochemistry , biology , paleontology , gene
This review highlights recent developments in glycosylation analysis by modern MS in combination with CE based preseparation. Focused on CE‐MS strategies aimed for glycotyping, the review addresses the detailed glycoform analysis of glycoproteins, glycopeptides, and proteoglycans. Glycoform analysis in the context of modern glycoproteomics is briefly addressed, as well. CZE, CIEF, and frontal analysis CE approaches hyphenated to high‐resolution multistage MS for the detailed analysis of protein‐linked glycan structures are overviewed in a comprehensive way. Advantages and limitations of various methodological approaches and techniques as well as mass spectrometric instrumentation are discussed in the particular context of glycoanalysis.