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Separation of multiphosphorylated peptide isomers by CZE
Author(s) -
Muetzelburg Marika V.,
Hoffmann Ralf
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800089
Subject(s) - phosphopeptide , chemistry , formic acid , electrolyte , peptide , chromatography , phosphate , aqueous solution , structural isomer , stereochemistry , biochemistry , organic chemistry , electrode
A separation of mono, doubly and triply phosphorylated isomers was developed with CZE with an aqueous electrolyte containing 3.9 mol/L formic acid and 30% v/v trifluoroethanol. Thus a mixture of ten phosphopeptides corresponding to the human tau sequence 226–240 was separated within 70 min. Although peptides with different phosphorylation degrees, i.e. 0–3 phosphate groups, were well separated, some of the phosphopeptide isomers containing one or two phosphate groups were only partially separated. The electrolyte system is compatible with both MALDI‐ and ESI‐MS, allowing a direct coupling, and thus could have some interesting applications in proteomics.