Premium
Coupling of solid‐phase microextraction continuous bed (monolithic) capillaries with capillary zone electrophoresis for direct analysis of drugs in biological fluids
Author(s) -
Jarmalavičienė Reda,
Szumski Michał,
Kornyšova Olga,
Kłodzińska Ewa,
Westerlund Douglas,
Krawczyk Stanislas,
Mickevičius Donatas,
Buszewski Bogusław,
Maruška Audrius
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800046
Subject(s) - solid phase microextraction , chromatography , capillary electrophoresis , capillary action , chemistry , analyte , sample preparation , analytical chemistry (journal) , capillary electrochromatography , materials science , mass spectrometry , gas chromatography–mass spectrometry , composite material
Hyperlink robust biocompatible solid‐phase microextraction (SPME) devices were prepared using continuous bed (monolithic) restricted‐access media (RAM) as the SPME capillary insert. The RAM‐based SPME approach was able to simultaneously separate proteins from a biological sample, while directly extracting the active components of caffeine, paracetamol and acetylsalicylic acid from the drug NeoCitramonum . The devices were interfaced with a CZE system and fully automated analysis for sample preconcentration, desorption, separation and quantification of analytes was evaluated. Comparative study of in‐line coupled SPME–CZE using RAM and RP capillary inserts was carried out. Using an SPME (RAM) insert, the calculated caffeine, paracetamol and acetylsalicylic acid LODs in a bovine plasma sample were 0.3, 0.8 and 1.9 ng/mL, respectively.