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Aptamers and molecularly imprinted polymers as artificial biomimetic receptors in affinity capillary electrophoresis and electrochromatography
Author(s) -
Giovannoli Cristina,
Baggiani Claudio,
Anfossi Laura,
Giraudi Gianfranco
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200800004
Subject(s) - molecularly imprinted polymer , aptamer , electrochromatography , molecular recognition , capillary electrophoresis , capillary electrochromatography , microscale chemistry , chemistry , molecular imprinting , polymer , affinity chromatography , affinity electrophoresis , nanotechnology , combinatorial chemistry , molecule , selectivity , materials science , chromatography , biochemistry , biology , organic chemistry , microbiology and biotechnology , mathematics education , mathematics , enzyme , catalysis
Abstract Artificial biomimetic receptors, such as aptamers and molecular‐imprinted polymers, show antibody‐like properties which are due to molecular recognition phenomena characterized by high affinity and selectivity. These binding features have made them suitable in all those application fields in which selective recognition is required. Thus, it is not surprising that they are finding applications in affinity CE as well. Recently, a variety of ACE methods have shown themselves to be suitable tools to provide a detailed quantitative characterization of the thermodynamic and kinetic aspects of binding. At the same time, affinity CE can exploit the peculiarities of these binding interactions to set up CE‐based analytical tools for the separation and the determination of specific target molecules in microscale formats. This review will provide a detailed description of affinity CE methods recently reported in the literature and related to these topics.