Premium
High‐sensitivity capillary electrophoresis for speciation of organomercury in biological samples using hollow fiber‐based liquid‐liquid‐liquid microextraction combined with on‐line preconcentration by large‐volume sample stacking
Author(s) -
Li Pingjing,
Duan Jiankun,
Hu Bin
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700920
Subject(s) - capillary electrophoresis , certified reference materials , chromatography , analytical chemistry (journal) , organomercury , fiber , chemistry , stacking , analyte , detection limit , electrolyte , materials science , electrode , organic chemistry
A hollow fiber‐based liquid‐liquid‐liquid microextraction (HF‐LLLME) combined with on‐line large‐volume sample stacking (LVSS) has been developed for the speciation of organomercury in biological samples by CE with UV detection. Separation was achieved in less than 11 min with an electrolyte consisting of 35 mM sodium tetraborate at pH 9.1. In LVSS, a reverse electrode polarity‐stacking mode (REPSM) was applied as on‐line preconcentration strategy. In HF‐LLLME, the analytes were extracted from 12 mL volume of sample solution (pH adjusted to 3.0) into bromobenzene impregnated in the pores of the hollow fiber, and into an acceptor solution of L ‐cysteine (15 μL, 0.02% w/v) inside the hollow fiber. Under the optimized conditions, concentration factors of 2610–4580 were achieved and LODs in the range of 0.03–0.14 μg/L were feasible. The linearity was found to be over two orders of magnitude with correlation coefficient of 0.9991–0.9996. The developed method has been validated using a certified reference material (DORM‐2, dogfish muscle), and the determined values coincided very well with the certified values. The method was also applied to the speciation of organomercury in three kinds of fish samples and human hair samples.