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Determination of flurbiprofen enantiomers in plasma using a single‐isomer amino cyclodextrin derivative in nonaqueous capillary electrophoresis
Author(s) -
Rousseau Anne,
Pedrini Matteo,
Chiap Patrice,
Ivanyi Robert,
Crommen Jacques,
Fillet Marianne,
Servais AnneCatherine
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700919
Subject(s) - flurbiprofen , capillary electrophoresis , enantiomer , chromatography , cyclodextrin , chemistry , derivative (finance) , capillary action , electrophoresis , plasma , stereochemistry , materials science , biology , pharmacology , physics , composite material , quantum mechanics , financial economics , economics
A nonaqueous capillary electrophoresis (NACE) assay was developed for the separation and determination of flurbiprofen enantiomers in plasma samples using 6‐monodeoxy‐6‐mono(3‐hydroxy)propylamino‐β‐cyclodextrin as chiral selector. The nonaqueous background electrolyte was made up of 40 mM ammonium acetate in methanol (MeOH), and flufenamic acid was employed as internal standard. Solid‐phase extraction was used for sample cleanup prior to the NACE separation. The NACE method reproducibility was optimized by evaluating different capillary washing sequences between runs. After having tested various conditions, trifluoroacetic acid (1 M) in MeOH was finally selected. Concerning the solid‐phase extraction procedure, good and reproducible analyte recoveries were obtained using MeOH for protein denaturation and a polymeric phase combining hydrophobic interactions with anion exchange properties (Oasis ® MAX) was selected as extraction sorbent. The method selectivity was not only demonstrated toward a blank plasma sample but also toward other non‐steroidal anti‐inflammatory drugs. The method was then successfully validated with respect to response function, trueness, precision, accuracy, linearity and limit of quantification.