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Quantification of single‐stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary electrophoresis – Part II
Author(s) -
Stettler Alexandra R.,
Chaurin Valérie,
Constable Edwin C.,
Housecroft Catherine E.,
Schwarz Maria A.
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700890
Subject(s) - oligonucleotide , chemistry , guanine , capillary electrophoresis , divalent , nucleic acid , metal , affinity electrophoresis , divalent metal , metal ions in aqueous solution , crystallography , nucleotide , dna , inorganic chemistry , chromatography , biochemistry , organic chemistry , affinity chromatography , gene , enzyme
The interactions between tetranucleotides or heptanucleotides and inorganic cations have been measured by affinity CZE. The variation in migration behavior with increasing concentrations of divalent cations (Ca 2+ , Mg 2+ and Ni 2+ ) in the running buffer was investigated and quantified by the calculation of binding constants for mononuclear and multinuclear interactions. In addition to these fundamental studies of binding equilibria, the effect of sequence and the position of the guanine transition metal‐binding site in the oligonucleotide have been investigated.