Influence of polyelectrolyte coating conditions on capillary coating stability and separation efficiency in capillary electrophoresis | Zendy

Nehmé Reine | Zendy; Perrin Catherine | Zendy; Cottet Hervé | Zendy; Blanchin Marie D. | Zendy; Fabre Huguette | Zendy

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Influence of polyelectrolyte coating conditions on capillary coating stability and separation efficiency in capillary electrophoresis

Author(s) -

Nehmé Reine,

Perrin Catherine,

Cottet Hervé,

Blanchin Marie D.,

Fabre Huguette

Publication year - 2008

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.200700886

Subject(s) - coating , repeatability , polyelectrolyte , capillary electrophoresis , capillary action , materials science , chromatography , adsorption , electrophoresis , polyelectrolyte adsorption , ionic strength , analytical chemistry (journal) , chemical engineering , chemistry , composite material , polymer , aqueous solution , organic chemistry , engineering

Polyelectrolytes are widely used in capillary electrophoresis as coating agents of silica capillaries to prevent adsorption phenomena and improve the repeatability of peptide and protein analysis. A systematic study of the coating experimental conditions has been carried out to optimize coating stability and performance. The main experimental parameters studied were the type and concentration of polyelectrolytes used in several monolayer and multilayer coatings, the ionic strength of coating and stabilizing solutions, and the procedures used for coating and capillary storage. Electroosmotic flow magnitude, direction and repeatability were used to monitor coating stability. Coating ability to limit adsorption was investigated by monitoring variations of migration times, time‐corrected peak areas and separation efficiency of test peptides. Capillary‐to‐capillary and batch‐to‐batch reproducibility was also studied. In addition, the separation performance of polyelectrolyte coatings were compared to those obtained with bare silica capillaries.

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