Premium
Critical evaluation of buffering solutions for p K a determination by capillary electrophoresis
Author(s) -
Fuguet Elisabet,
Reta Mario,
Gibert Carme,
Rosés Martí,
Bosch Elisabeth,
Ràfols Clara
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700869
Subject(s) - boric acid , chemistry , hepes , tris , ammonium acetate , acetic acid , capillary electrophoresis , pyridine , analyte , phosphate , ammonia , ammonium , boron , chromatography , inorganic chemistry , nuclear chemistry , organic chemistry , biochemistry , high performance liquid chromatography
The performance of the most common and also some other less common CE buffers has been tested for the p K a determination of several types of compounds (pyridine, amines, and phenols). The selected buffers cover a pH ranging from 3.7 to 11.8. Whereas some buffers, like acetic acid/acetate, BisTrisH + /BisTris, TrisH + /Tris, CHES/CHES − , and CAPS/CAPS − can be used with all type of analytes, others like ammonium/ammonia, butylammonium/butylammonia, ethylammonium/ethylammonia, diethylammonium/diethylammonia, and hydrogenphosphate/phosphate are not recommended because they interact with a wide range of compounds. The rest of the tested buffers (dihydrogenphosphate/hydrogenphosphate, MES/MES − , HEPES/HEPES − , and boric acid/borate) can show specific interactions depending on the nature of the analytes, and their use in some applications should be restricted.