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Pattern recognition in capillary electrophoresis data using dynamic programming in the wavelet domain
Author(s) -
Ceballos Gerardo A.,
Paredes Jose L.,
Hernández Luis F.
Publication year - 2008
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200700831
Subject(s) - electropherogram , pattern recognition (psychology) , artificial intelligence , wavelet , computer science , preprocessor , computer vision , capillary electrophoresis , chemistry , chromatography
A novel approach for CE data analysis based on pattern recognition techniques in the wavelet domain is presented. Low‐resolution, denoised electropherograms are obtained by applying several preprocessing algorithms including denoising, baseline correction, and detection of the region of interest in the wavelet domain. The resultant signals are mapped into character sequences using first derivative information and multilevel peak height quantization. Next, a local alignment algorithm is applied on the coded sequences for peak pattern recognition. We also propose 2‐D and 3‐D representations of the found patterns for fast visual evaluation of the variability of chemical substances concentration in the analyzed samples. The proposed approach is tested on the analysis of intracerebral microdialysate data obtained by CE and LIF detection, achieving a correct detection rate of about 85% with a processing time of less than 0.3 s per 25 000‐point electropherogram. Using a local alignment algorithm on low‐resolution denoised electropherograms might have a great impact on high‐throughput CE since the proposed methodology will substitute automatic fast pattern recognition analysis for slow, human based time‐consuming visual pattern recognition methods.